Rapid establishment of laboratory diagnostics for the novel coronavirus SARS-CoV-2 in Bavaria, Germany, February 2020
Identifieur interne : 000418 ( an2020/Analysis ); précédent : 000417; suivant : 000419Rapid establishment of laboratory diagnostics for the novel coronavirus SARS-CoV-2 in Bavaria, Germany, February 2020
Auteurs : Regina Konrad [Allemagne] ; Ute Eberle [Allemagne] ; Alexandra Dangel [Allemagne] ; Bianca Treis [Allemagne] ; Anja Berger [Allemagne] ; Katja Bengs [Allemagne] ; Volker Fingerle [Allemagne] ; Bernhard Liebl [Allemagne] ; Nikolaus Ackermann [Allemagne] ; Andreas Sing [Allemagne]Source :
- Eurosurveillance [ 1025-496X ] ; 2020.
Descripteurs français
- KwdFr :
- Allemagne, Facteurs temps, Flux de travaux, Humains, Infections à coronavirus (diagnostic), Infections à coronavirus (génétique), Pneumopathie virale (diagnostic), Pneumopathie virale (génétique), Protéines de l'enveloppe virale (analyse), Protéines de l'enveloppe virale (génétique), Réaction de polymérisation en chaine en temps réel (), Sensibilité et spécificité, Séquençage par oligonucléotides en batterie.
- MESH :
- analyse : Protéines de l'enveloppe virale.
- diagnostic : Infections à coronavirus, Pneumopathie virale.
- génétique : Infections à coronavirus, Pneumopathie virale, Protéines de l'enveloppe virale.
- Allemagne, Facteurs temps, Flux de travaux, Humains, Réaction de polymérisation en chaine en temps réel, Sensibilité et spécificité, Séquençage par oligonucléotides en batterie.
- Wicri :
- geographic : Allemagne.
English descriptors
- KwdEn :
- Betacoronavirus (genetics), Betacoronavirus (isolation & purification), Coronavirus Infections (diagnosis), Coronavirus Infections (genetics), Germany, Humans, Oligonucleotide Array Sequence Analysis, Pneumonia, Viral (diagnosis), Pneumonia, Viral (genetics), Real-Time Polymerase Chain Reaction (methods), Sensitivity and Specificity, Time Factors, Viral Envelope Proteins (analysis), Viral Envelope Proteins (genetics), Workflow.
- MESH :
- chemical , analysis : Viral Envelope Proteins.
- chemical , genetics : Viral Envelope Proteins.
- geographic : Germany.
- diagnosis : Coronavirus Infections, Pneumonia, Viral.
- genetics : Betacoronavirus, Coronavirus Infections, Pneumonia, Viral.
- isolation & purification : Betacoronavirus.
- methods : Real-Time Polymerase Chain Reaction.
- Humans, Oligonucleotide Array Sequence Analysis, Sensitivity and Specificity, Time Factors, Workflow.
Abstract
The need for timely establishment of diagnostic assays arose when Germany was confronted with the first travel-associated outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Europe. We describe our laboratory experiences during a large contact tracing investigation, comparing previously published real-time RT-PCR assays in different PCR systems and a commercial kit. We found that assay performance using the same primers and probes with different PCR systems varied and the commercial kit performed well.
Url:
DOI: 10.2807/1560-7917.ES.2020.25.9.2000173
PubMed: 32156330
PubMed Central: 7068163
Affiliations:
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PMC:7068163Le document en format XML
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xml:lang="fr">Allemagne</country><wicri:regionArea>Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea><wicri:noRegion>Oberschleißheim</wicri:noRegion><wicri:noRegion>Oberschleißheim</wicri:noRegion><wicri:noRegion>Oberschleißheim</wicri:noRegion></affiliation><affiliation><nlm:aff id="aff2">These authors contributed equally to this work</nlm:aff></affiliation></author><author><name sortKey="Eberle, Ute" sort="Eberle, Ute" uniqKey="Eberle U" first="Ute" last="Eberle">Ute Eberle</name><affiliation><nlm:aff id="aff2">These authors contributed equally to this work</nlm:aff></affiliation><affiliation wicri:level="1"><nlm:aff id="aff3">Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea><wicri:noRegion>Oberschleißheim</wicri:noRegion><wicri:noRegion>Oberschleißheim</wicri:noRegion><wicri:noRegion>Oberschleißheim</wicri:noRegion></affiliation></author><author><name sortKey="Dangel, Alexandra" sort="Dangel, Alexandra" uniqKey="Dangel A" first="Alexandra" last="Dangel">Alexandra Dangel</name><affiliation wicri:level="1"><nlm:aff id="aff1">Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea><wicri:noRegion>Oberschleißheim</wicri:noRegion><wicri:noRegion>Oberschleißheim</wicri:noRegion><wicri:noRegion>Oberschleißheim</wicri:noRegion></affiliation><affiliation><nlm:aff id="aff2">These authors contributed equally to this work</nlm:aff></affiliation></author><author><name sortKey="Treis, Bianca" sort="Treis, Bianca" uniqKey="Treis B" first="Bianca" last="Treis">Bianca Treis</name><affiliation wicri:level="1"><nlm:aff id="aff3">Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea><wicri:noRegion>Oberschleißheim</wicri:noRegion><wicri:noRegion>Oberschleißheim</wicri:noRegion><wicri:noRegion>Oberschleißheim</wicri:noRegion></affiliation></author><author><name sortKey="Berger, Anja" sort="Berger, Anja" uniqKey="Berger A" first="Anja" last="Berger">Anja Berger</name><affiliation wicri:level="1"><nlm:aff id="aff1">Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea><wicri:noRegion>Oberschleißheim</wicri:noRegion><wicri:noRegion>Oberschleißheim</wicri:noRegion><wicri:noRegion>Oberschleißheim</wicri:noRegion></affiliation></author><author><name sortKey="Bengs, Katja" sort="Bengs, Katja" uniqKey="Bengs K" first="Katja" last="Bengs">Katja Bengs</name><affiliation wicri:level="1"><nlm:aff id="aff1">Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea><wicri:noRegion>Oberschleißheim</wicri:noRegion><wicri:noRegion>Oberschleißheim</wicri:noRegion><wicri:noRegion>Oberschleißheim</wicri:noRegion></affiliation></author><author><name sortKey="Fingerle, Volker" sort="Fingerle, Volker" uniqKey="Fingerle V" first="Volker" last="Fingerle">Volker Fingerle</name><affiliation wicri:level="1"><nlm:aff id="aff3">Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea><wicri:noRegion>Oberschleißheim</wicri:noRegion><wicri:noRegion>Oberschleißheim</wicri:noRegion><wicri:noRegion>Oberschleißheim</wicri:noRegion></affiliation></author><author><name sortKey="Liebl, Bernhard" sort="Liebl, Bernhard" uniqKey="Liebl B" first="Bernhard" last="Liebl">Bernhard Liebl</name><affiliation wicri:level="1"><nlm:aff id="aff4">State Institute of Health, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>State Institute of Health, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea><wicri:noRegion>Oberschleißheim</wicri:noRegion><wicri:noRegion>Oberschleißheim</wicri:noRegion><wicri:noRegion>Oberschleißheim</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:aff id="aff5">Ludwig Maximilians-Universität, Munich, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Ludwig Maximilians-Universität, Munich</wicri:regionArea><placeName><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Haute-Bavière</region><settlement type="city">Munich</settlement></placeName></affiliation></author><author><name sortKey="Ackermann, Nikolaus" sort="Ackermann, Nikolaus" uniqKey="Ackermann N" first="Nikolaus" last="Ackermann">Nikolaus Ackermann</name><affiliation><nlm:aff id="aff2">These authors contributed equally to this work</nlm:aff></affiliation><affiliation wicri:level="1"><nlm:aff id="aff3">Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea><wicri:noRegion>Oberschleißheim</wicri:noRegion><wicri:noRegion>Oberschleißheim</wicri:noRegion><wicri:noRegion>Oberschleißheim</wicri:noRegion></affiliation></author><author><name sortKey="Sing, Andreas" sort="Sing, Andreas" uniqKey="Sing A" first="Andreas" last="Sing">Andreas Sing</name><affiliation wicri:level="1"><nlm:aff id="aff1">Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim</wicri:regionArea><wicri:noRegion>Oberschleißheim</wicri:noRegion><wicri:noRegion>Oberschleißheim</wicri:noRegion><wicri:noRegion>Oberschleißheim</wicri:noRegion></affiliation><affiliation><nlm:aff id="aff2">These authors contributed equally to this work</nlm:aff></affiliation><affiliation wicri:level="3"><nlm:aff id="aff5">Ludwig Maximilians-Universität, Munich, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Ludwig Maximilians-Universität, Munich</wicri:regionArea><placeName><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Haute-Bavière</region><settlement type="city">Munich</settlement></placeName></affiliation></author></analytic><series><title level="j">Eurosurveillance</title><idno type="ISSN">1025-496X</idno><idno type="eISSN">1560-7917</idno><imprint><date when="2020">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Betacoronavirus (genetics)</term><term>Betacoronavirus (isolation & purification)</term><term>Coronavirus Infections (diagnosis)</term><term>Coronavirus Infections 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xml:lang="fr"><term>Allemagne</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The need for timely establishment of diagnostic assays arose when Germany was confronted with the first travel-associated outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Europe. We describe our laboratory experiences during a large contact tracing investigation, comparing previously published real-time RT-PCR assays in different PCR systems and a commercial kit. We found that assay performance using the same primers and probes with different PCR systems varied and the commercial kit performed well. </p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Rothe, C" uniqKey="Rothe C">C Rothe</name></author><author><name sortKey="Schunk, M" uniqKey="Schunk M">M Schunk</name></author><author><name sortKey="Sothmann, P" uniqKey="Sothmann P">P Sothmann</name></author><author><name sortKey="Bretzel, G" uniqKey="Bretzel G">G Bretzel</name></author><author><name sortKey="Froeschl, G" uniqKey="Froeschl G">G Froeschl</name></author><author><name sortKey="Wallrauch, C" uniqKey="Wallrauch C">C Wallrauch</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Corman, Vm" uniqKey="Corman V">VM Corman</name></author><author><name sortKey="Landt, O" uniqKey="Landt O">O Landt</name></author><author><name sortKey="Kaiser, M" uniqKey="Kaiser M">M Kaiser</name></author><author><name sortKey="Molenkamp, R" uniqKey="Molenkamp R">R Molenkamp</name></author><author><name sortKey="Meijer, A" uniqKey="Meijer A">A Meijer</name></author><author><name sortKey="Chu, Dkw" uniqKey="Chu D">DKW Chu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Romette, Jl" uniqKey="Romette J">JL Romette</name></author><author><name sortKey="Prat, Cm" uniqKey="Prat C">CM Prat</name></author><author><name sortKey="Gould, Ea" uniqKey="Gould E">EA Gould</name></author><author><name sortKey="De Lamballerie, X" uniqKey="De Lamballerie X">X de Lamballerie</name></author><author><name sortKey="Charrel, R" uniqKey="Charrel R">R Charrel</name></author><author><name sortKey="Coutard, B" uniqKey="Coutard B">B Coutard</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hummel, Kb" uniqKey="Hummel K">KB Hummel</name></author><author><name sortKey="Lowe, L" uniqKey="Lowe L">L Lowe</name></author><author><name sortKey="Bellini, Wj" uniqKey="Bellini W">WJ Bellini</name></author><author><name sortKey="Rota, Pa" uniqKey="Rota P">PA Rota</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Ren, Ll" uniqKey="Ren L">LL Ren</name></author><author><name sortKey="Wang, Ym" uniqKey="Wang Y">YM Wang</name></author><author><name sortKey="Wu, Zq" uniqKey="Wu Z">ZQ Wu</name></author><author><name sortKey="Xiang, Zc" uniqKey="Xiang Z">ZC Xiang</name></author><author><name sortKey="Guo, L" uniqKey="Guo L">L Guo</name></author><author><name sortKey="Xu, T" uniqKey="Xu T">T Xu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wu, F" uniqKey="Wu F">F Wu</name></author><author><name sortKey="Zhao, S" uniqKey="Zhao S">S Zhao</name></author><author><name sortKey="Yu, B" uniqKey="Yu B">B Yu</name></author><author><name sortKey="Chen, Ym" uniqKey="Chen Y">YM Chen</name></author><author><name sortKey="Wang, W" uniqKey="Wang W">W Wang</name></author><author><name sortKey="Song, Zg" uniqKey="Song Z">ZG Song</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Reusken, Cbem" uniqKey="Reusken C">CBEM Reusken</name></author><author><name sortKey="Broberg, Ek" uniqKey="Broberg E">EK Broberg</name></author><author><name sortKey="Haagmans, B" uniqKey="Haagmans B">B Haagmans</name></author><author><name sortKey="Meijer, A" uniqKey="Meijer A">A Meijer</name></author><author><name sortKey="Corman, Vm" uniqKey="Corman V">VM Corman</name></author><author><name sortKey="Papa, A" uniqKey="Papa A">A Papa</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Allemagne</li></country><region><li>Bavière</li><li>District de Haute-Bavière</li></region><settlement><li>Munich</li></settlement></list><tree><country name="Allemagne"><noRegion><name sortKey="Konrad, Regina" sort="Konrad, Regina" uniqKey="Konrad R" first="Regina" last="Konrad">Regina Konrad</name></noRegion><name sortKey="Ackermann, Nikolaus" sort="Ackermann, Nikolaus" uniqKey="Ackermann N" first="Nikolaus" last="Ackermann">Nikolaus Ackermann</name><name sortKey="Bengs, Katja" sort="Bengs, Katja" uniqKey="Bengs K" first="Katja" last="Bengs">Katja Bengs</name><name sortKey="Berger, Anja" sort="Berger, Anja" uniqKey="Berger A" first="Anja" last="Berger">Anja Berger</name><name sortKey="Dangel, Alexandra" sort="Dangel, Alexandra" uniqKey="Dangel A" first="Alexandra" last="Dangel">Alexandra Dangel</name><name sortKey="Eberle, Ute" sort="Eberle, Ute" uniqKey="Eberle U" first="Ute" last="Eberle">Ute Eberle</name><name sortKey="Fingerle, Volker" sort="Fingerle, Volker" uniqKey="Fingerle V" first="Volker" last="Fingerle">Volker Fingerle</name><name sortKey="Liebl, Bernhard" sort="Liebl, Bernhard" uniqKey="Liebl B" first="Bernhard" last="Liebl">Bernhard Liebl</name><name sortKey="Liebl, Bernhard" sort="Liebl, Bernhard" uniqKey="Liebl B" first="Bernhard" last="Liebl">Bernhard Liebl</name><name sortKey="Sing, Andreas" sort="Sing, Andreas" uniqKey="Sing A" first="Andreas" last="Sing">Andreas Sing</name><name sortKey="Sing, Andreas" sort="Sing, Andreas" uniqKey="Sing A" first="Andreas" last="Sing">Andreas Sing</name><name sortKey="Treis, Bianca" sort="Treis, Bianca" uniqKey="Treis B" first="Bianca" last="Treis">Bianca Treis</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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